• Product description
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NanoGel dT20 was designed to address the selectivity and capacity requirements for large-scale downstream purification of a range of mRNA constructs used in vaccine and gene therapy applications. It can selectively captures the mRNA via the polyadenylated (polyA) tail using simple purification steps of salt for binding and water for elution. NanoGel dT20 effectively separates mRNA from components of the transcription reaction process, such as enzymes and plasmid DNA. It allows for a robust platform and efficient purification process for a range of mRNA molecules and applications.

  • Monodisperse matrix of very wide pores provides high binding capacity

  • Simplified workflow, high efficiency

  • Excellent scalability



Product Name

NanoGel dT20

Chromatography Technique

mRNA affinity

Matrix

PS/DVB

Particle Size

50 µm

Ligand

dT-20mer

Dynamic Binding Capacity

~ 2 mg·mL-1 (mRNA)

Maximum Pressure

3 MPa

Clean In Place

0.1 M NaOH

Recommended Flow Velocity

50-300 cm/h

pH Stability

2-13

Chemical Stability

Stable in commonly used buffers, 2 M HAc, 1 M HCl, 100% ethanol, isopropyl alcohol, etc. Avoid long-term exposure to strong oxidant, benzyl alcohol or tetrahydrofuran.

Operational Temperature

2-65

Storage

20% Ethanol, 2-8



Product Name

Pack Size

Cat. No.

NanoGel dT20 10mL

10mL

17030-050150-2010

NanoGel dT20 30mL

30mL

17030-050150-2030

NanoGel dT20 50mL

50mL

17030-050150-2050

NanoGel dT20 100mL

100mL

17030-050150-2100

NanoGel dT20 300mL

300mL

17030-050150-2300

NanoGel dT20 500mL

500mL

17030-050150-2500

NanoGel dT20 1L

1L

17030-050150-1001

NanoGel dT20 5L

5L

17030-050150-1005

NanoGel dT20 10L

10L

17030-050150-1010

NanoGel dT20 50L

50L

17030-050150-1050

NanoGel dT20 100L

100L

17030-050150-1100