Ceramic hydroxyapatite (CHT) is a hydroxyapatite-based composite material with important application value as a chromatographic media. It has a spherical appearance and macropores. Unlike traditional chromatographic media, CHT is a mixed-mode resin with both metal affinity and cation ion exchange capabilities offers a unique selectivity that can provide unique and effective purification solutions. CHT chromatography media can be used for the purification of various types of biomacromolecules, including: antibodies, virus particles, vaccines, recombinant proteins and nucleic acid, etc.
NMCHT Type I has a higher binding capacity and is suitable for small protein purification. NMCHT Type II has a larger pore size and is more suitable for the purification of macromolecules such as viruses, IgM, VLP particles, plasmids, etc.
Product name | NMCHT Type I | NMCHT Type II |
Functional group | Ca2+, PO43-, -OH | |
Particle size | 40±4 μm | |
Tap-settled density | 0.72 g/ml | |
DBC(IgG) | ~35 mg/ml | ~15 mg/ml |
Recommended flow rate | 50-1000 cm/h | |
Sanitization | 1-2 M NaOH | |
pH stability | 6.5-14, at least 1year in 1 M NaOH | |
Regeneration | 0.4 M sodium phosphate buffer,pH 7-7.5; 1 M trisodium phosphate,pH 11-12; If a higher phosphate concentration is required, please use 0.4-1 M potassium phosphate buffer | |
Autoclavability | 121 ℃,20 min, phosphate buffer, pH 7. | |
Storage* | 0.1 M NaOH, room temperature |
* Please store the product under sealed, room temperature(2-30℃), and dry conditions in original packaging. Here are the recommended storage conditions once the package has been opened.
Product name | Package | Cat. No. |
NMCHT Type I | 10 g | 80000-040001-4010 |
100 g | 80000-040001-4100 | |
1 KG | 80000-040001-3001 | |
5 KG | 80000-040001-3005 | |
NMCHT Type II | 10 g | 80000-040002-4010 |
100 g | 80000-040002-4100 | |
1 KG | 80000-040002-3001 | |
5 KG | 80000-040002-3005 |