Heparin is a linear and highly sulfated glycosaminoglycan which has anti-coagulant properties. Due to its polyanionic nature, heparin interacts with a wide range of biomolecules including plasma coagulation proteins, lipoprotein lipase, collagenase, and DNA polymerase. Immobilized heparin is widely used as an adsorbent in affinity chromatography for the purification of biological substances.
NanoMicro offers two Heparin affinity resins, UniGel-65Heparin and NM90Agarose Heparin, with different matrices. UniGel-65Heparin has monodisperse polymethacrylate matrix of UniGel, and hence provides superior mechanic strength for fast flow operation. Due to its overall robust performance, UniGel-65Heparin is a good choice not only for bioprocessing heparin affinity chromatography resin but also for sample preparation sorbent.
NM90 Agarose Heparin is based on crosslinked agarose and has competitive binding capacity. NM90Agarose Heparin is a good alternative choice when high flow rate is not needed in the chromatography process .
Product Name | UniGel®-65Heparin | NM90Agarose Heparin |
Chromatography technique | Heparin affinity | Heparin affinity |
Matrix | Polymethacrylate | Cross-linked agarose |
Particle Size | 65 μm | 90 μm |
Ligand | Heparin | Heparin |
Dynamic Binding Capacity | ~ 5 mg·mL-1 (ATIII) | ~ 5 mg·mL-1 (ATIII) |
Maximum Pressure | 0.8 MPa | 0.3 MPa |
Clean In Place | 2 M NaCl | 2 M NaCl |
Recommended Flow Velocity | 100-500 cm/h | 100-500 cm/h |
pH Stability | 4-10 | 4-12 |
Chemical Stability | Stable in commonly aqueous used buffers, 0.1 M NaOH, 0.05 M NaAc, 8 M urea, 6 M guanidine hydrochloride, non-ionic detergent, 10% glycerin etc. | Stable in commonly aqueous used buffers, 0.1 M NaOH, 0.05 M NaAc, 8 M urea, 6 M guanidine hydrochloride, non-ionic detergent, 10% glycerin etc. |
Operational Temperature | 2-40 ℃ | |
Storage | 20% Ethanol, 4-25 ℃ | 20% Ethanol, 4-25 ℃ |
Product Name | Pack Size | Cat. No. |
UniGel-65Heparin 10mL | 10mL | 17012-065100-2010 |
UniGel-65Heparin 30mL | 30mL | 17012-065100-2030 |
UniGel-65Heparin 50mL | 50mL | 17012-065100-2050 |
UniGel-65Heparin 100mL | 100mL | 17012-065100-2100 |
UniGel-65Heparin 300mL | 300mL | 17012-065100-2300 |
UniGel-65Heparin 500mL | 500mL | 17012-065100-2500 |
UniGel-65Heparin 1L | 1L | 17012-065100-1001 |
UniGel-65Heparin 5L | 5L | 17012-065100-1005 |
UniGel-65Heparin 10L | 10L | 17012-065100-1010 |
UniGel-65Heparin 50L | 50L | 17012-065100-1050 |
UniGel-65Heparin 100L | 100L | 17012-065100-1100 |
NM90Agarose Heparin 10mL | 10mL | 17012-090001-2010 |
NM90Agarose Heparin 30mL | 30mL | 17012-090001-2030 |
NM90Agarose Heparin 50mL | 50mL | 17012-090001-2050 |
NM90Agarose Heparin 100mL | 100mL | 17012-090001-2100 |
NM90Agarose Heparin 300mL | 300mL | 17012-090001-2300 |
NM90Agarose Heparin 500mL | 500mL | 17012-090001-2500 |
NM90Agarose Heparin 1L | 1L | 17012-090001-1001 |
NM90Agarose Heparin 5L | 5L | 17012-090001-1005 |
NM90Agarose Heparin 10L | 10L | 17012-090001-1010 |
NM90Agarose Heparin 50L | 50L | 17012-090001-1050 |
NM90Agarose Heparin 100L | 100L | 17012-090001-1100 |