Chromatography Technique

Antibody affinity

Antibody affinity

Matrix

Cross-linked agarose

Cross-linked agarose

Particle Size

90 μm

69 μm

Ligand

Alkaline-stabilized recombinant protein A

Alkaline-stabilized recombinant protein A

Dynamic Binding Capacity

55 mg·mL^-1 (Human IgG, 6 min residence time)

＞65 mg·mL^-1 (Human IgG, 6 min residence time)

Maximum Pressure

0.3 MPa

0.3 MPa

Clean in Place

0.1-0.5 M NaOH

0.1-0.5 M NaOH

Recommended Flow Velocity

100-500 cm/h

100-500 cm/h

pH Stability

2-12

2-12

Chemical Stability

All commonly used buffers, 10 mM hydrochloric acid, 0.1 M citric acid (pH3), 6M urea, 6M guanidine hydrochloride, 30% isopropyl alcohol, 20% ethanol.

All commonly used buffers, 10 mM hydrochloric acid, 0.1 M citric acid (pH3), 6M urea, 6M guanidine hydrochloride, 30% isopropyl alcohol, 20% ethanol.

Operational Temperature

2-40 ℃

2-40 ℃

Storage

20% Ethanol, 2-8 ℃

20% Ethanol, 2-8 ℃