Hydrophobic interaction chromatography (HIC) is a technology that utilizes the differences in the hydrophilicity and hydrophobicity of biological macromolecules to achieve separation. Under high-salt conditions, biological macromolecules are adsorbed on the hydrophobic groups of the hydrophobic chromatography medium. By reducing the salt concentration, different proteins are eluted in order from weak to strong hydrophobicity, so as to achieve the purpose of separation and purification. With mild operating conditions and high resolution, HIC is one of the commonly used methods for the separation and purification of biological macromolecules.
UniHR HIC resins are based on monodisperse, hydrophilically modified polymethacrylate matrix. Phenyl and butyl hydrophobic groups are bonded onto this hydrophilic matrix resin to respectively provide two families of HIC resins .
Uniformed particle size
High mass transfer and high resolution
Outstanding mechanic strength and superior pressure-flow characteristics
Minimal non-specific binding and high recovery
We now offer UniHR Butyl-30S / UniHR Phenyl-30L /UniHR Butyl-30L / UniHR Phenyl-30L / UniHR Butyl-60S / UniHR Phenyl-60S / UniHR Butyl-80L / UniHR Phenyl-80L.
Butyl phase
Product Name | UniHR Butyl-30S | UniHR Butyl-30L | UniHR Butyl-60S | UniHR Butyl-80L |
Chromatography Technique | Hydrophobic interaction | Hydrophobic interaction | Hydrophobic interaction | Hydrophobic interaction |
Matrix | Polymethacrylate | Polymethacrylate | Polymethacrylate | Polymethacrylate |
Particle Size | 35 µm | 32 µm | 60 µm | 80 µm |
Ligand | Butyl | Butyl | Butyl | Butyl |
Dynamic Binding Capacity | ~23 mg·mL-1 (Lys) | ~15 mg·mL-1 (Lys) | ~20 mg·mL-1 (Lys) | ~10 mg·mL-1 (Lys) |
Maximum Pressure | 1.0 MPa | 1.0 MPa | 0.8 MPa | 0.5 MPa |
Clean In Place | 1 M NaOH / 70% Ethanol | 1 M NaOH / 70% Ethanol | 1 M NaOH / 70% Ethanol | 1 M NaOH / 70% Ethanol |
Recommended Flow Velocity | 50-300 cm/h | 50-300 cm/h | 100-500 cm/h | 150-750 cm/h |
pH Stability | 2-12 | 2-12 | 2-12 | 2-12 |
Chemical Stability | Stable in commonly used buffers, 1 M HAc, 1 M NaOH, 1 M HCl, 70% ethanol, 30% isopropyl alcohol, 30% acetonitrile,1% SDS, 6 M guanidine hydrochloride, 8 M urea, etc. Avoid long-term exposure to strong oxidant. | |||
Operational Temperature | 15-30 ℃ | 15-30 ℃ | 15-30 ℃ | 15-30 ℃ |
Storage | 20% Ethanol，4-25 ℃ | 20% Ethanol，4-25 ℃ | 20% Ethanol，4-25 ℃ | 20% Ethanol，4-25 ℃ |
Phenyl phase
Product Name | UniHR Phenyl-30S | UniHR Phenyl-30L | UniHR Phenyl-60S | UniHR Phenyl-80L |
Chromatography Technique | Hydrophobic interaction | Hydrophobic interaction | Hydrophobic interaction | Hydrophobic interaction |
Matrix | Polymethacrylate | Polymethacrylate | Polymethacrylate | Polymethacrylate |
Particle Size | 35 µm | 32 µm | 60 µm | 80 µm |
Ligand | Phenyl | Phenyl | Phenyl | Phenyl |
Dynamic Binding Capacity | ~30 mg·mL-1 (Lys) | ~15 mg·mL-1 (Lys) | ~20 mg·mL-1 (Lys) | ~10 mg·mL-1 (Lys) |
Maximum Pressure | 1.0 MPa | 1.0 MPa | 0.8 MPa | 0.5 MPa |
Clean in Place | 1 M NaOH / 70% Ethanol | 1 M NaOH / 70% Ethanol | 1 M NaOH / 70% Ethanol | 1 M NaOH / 70% Ethanol |
Recommended Flow Velocity | 50-300 cm/h | 50-300 cm/h | 100-500 cm/h | 150-750 cm/h |
pH Stability | 2-12 | 2-12 | 2-12 | 2-12 |
Chemical Stability | Stable in commonly used buffers, 1 M HAc, 1 M NaOH, 1 M HCl, 70% ethanol, 30% isopropyl alcohol, 30% acetonitrile,1% SDS, 6 M guanidine hydrochloride, 8 M urea, etc. Avoid long-term exposure to strong oxidant. | |||
Operational Temperature | 15-30 ℃ | 15-30 ℃ | 15-30 ℃ | 15-30 ℃ |
Storage | 20% Ethanol，4-25 ℃ | 20% Ethanol，4-25 ℃ | 20% Ethanol，4-25 ℃ | 20% Ethanol，4-25 ℃ |
Product Name | Pack Size | Cat. No. |
UniHR Butyl-30S 10mL | 10mL | 06132-030050-2010 |
UniHR Butyl-30S 30mL | 30mL | 06132-030050-2030 |
UniHR Butyl-30S 50mL | 50mL | 06132-030050-2050 |
UniHR Butyl-30S 100mL | 100mL | 06132-030050-2100 |
UniHR Butyl-30S 300mL | 300mL | 06132-030050-2300 |
UniHR Butyl-30S 500mL | 500mL | 06132-030050-2500 |
UniHR Butyl-30S 1L | 1L | 06132-030050-1001 |
UniHR Butyl-30S 5L | 5L | 06132-030050-1005 |
UniHR Butyl-30S 10L | 10L | 06132-030050-1010 |
UniHR Butyl-30S 50L | 50L | 06132-030050-1050 |
UniHR Butyl-30S 100L | 100L | 06132-030050-1100 |
UniHR Phenyl-30S 10mL | 10mL | 06131-030050-2010 |
UniHR Phenyl-30S 30mL | 30mL | 06131-030050-2030 |
UniHR Phenyl-30S 50mL | 50mL | 06131-030050-2050 |
UniHR Phenyl-30S 100mL | 100mL | 06131-030050-2100 |
UniHR Phenyl-30S 300mL | 300mL | 06131-030050-2300 |
UniHR Phenyl-30S 500mL | 500mL | 06131-030050-2500 |
UniHR Phenyl-30S 1L | 1L | 06131-030050-1001 |
UniHR Phenyl-30S 5L | 5L | 06131-030050-1005 |
UniHR Phenyl-30S 10L | 10L | 06131-030050-1010 |
UniHR Phenyl-30S 50L | 50L | 06131-030050-1050 |
UniHR Phenyl-30S 100L | 100L | 06131-030050-1100 |
UniHR Butyl-30L 10mL | 10mL | 06132-030100-2010 |
UniHR Butyl-30L 30mL | 30mL | 06132-030100-2030 |
UniHR Butyl-30L 50mL | 50mL | 06132-030100-2050 |
UniHR Butyl-30L 100mL | 100mL | 06132-030100-2100 |
UniHR Butyl-30L 300mL | 300mL | 06132-030100-2300 |
UniHR Butyl-30L 500mL | 500mL | 06132-030100-2500 |
UniHR Butyl-30L 1L | 1L | 06132-030100-1001 |
UniHR Butyl-30L 5L | 5L | 06132-030100-1005 |
UniHR Butyl-30L 10L | 10L | 06132-030100-1010 |
UniHR Butyl-30L 50L | 50L | 06132-030100-1050 |
UniHR Butyl-30L 100L | 100L | 06132-030100-1100 |
UniHR Phenyl-30L 10mL | 10mL | 06131-030100-2010 |
UniHR Phenyl-30L 30mL | 30mL | 06131-030100-2030 |
UniHR Phenyl-30L 50mL | 50mL | 06131-030100-2050 |
UniHR Phenyl-30L 100mL | 100mL | 06131-030100-2100 |
UniHR Phenyl-30L 300mL | 300mL | 06131-030100-2300 |
UniHR Phenyl-30L 500mL | 500mL | 06131-030100-2500 |
UniHR Phenyl-30L 1L | 1L | 06131-030100-1001 |
UniHR Phenyl-30L 5L | 5L | 06131-030100-1005 |
UniHR Phenyl-30L 10L | 10L | 06131-030100-1010 |
UniHR Phenyl-30L 50L | 50L | 06131-030100-1050 |
UniHR Phenyl-30L 100L | 100L | 06131-030100-1100 |
UniHR Butyl-60S 10mL | 10mL | 06131-030100-2010 |
UniHR Butyl-60S 30mL | 30mL | 06131-030100-2030 |
UniHR Butyl-60S 50mL | 50mL | 06131-030100-2050 |
UniHR Butyl-60S 100mL | 100mL | 06131-030100-2100 |
UniHR Butyl-60S 300mL | 300mL | 06131-030100-2300 |
UniHR Butyl-60S 500mL | 500mL | 06131-030100-2500 |
UniHR Butyl-60S 1L | 1L | 06131-030100-1001 |
UniHR Butyl-60S 5L | 5L | 06131-030100-1005 |
UniHR Butyl-60S 10L | 10L | 06131-030100-1010 |
UniHR Butyl-60S 50L | 50L | 06131-030100-1050 |
UniHR Butyl-60S 100L | 100L | 06131-030100-1100 |
UniHR Phenyl-60S 10mL | 10mL | 06131-060050-2010 |
UniHR Phenyl-60S 30mL | 30mL | 06131-060050-2030 |
UniHR Phenyl-60S 50mL | 50mL | 06131-060050-2050 |
UniHR Phenyl-60S 100mL | 100mL | 06131-060050-2100 |
UniHR Phenyl-60S 300mL | 300mL | 06131-060050-2300 |
UniHR Phenyl-60S 500mL | 500mL | 06131-060050-2500 |
UniHR Phenyl-60S 1L | 1L | 06131-060050-1001 |
UniHR Phenyl-60S 5L | 5L | 06131-060050-1005 |
UniHR Phenyl-60S 10L | 10L | 06131-060050-1010 |
UniHR Phenyl-60S 50L | 50L | 06131-060050-1050 |
UniHR Phenyl-60S 100L | 100L | 06131-060050-1100 |
UniHR Butyl-80L 10mL | 10mL | 06132-080100-2010 |
UniHR Butyl-80L 30mL | 30mL | 06132-080100-2030 |
UniHR Butyl-80L 50mL | 50mL | 06132-080100-2050 |
UniHR Butyl-80L 100mL | 100mL | 06132-080100-2100 |
UniHR Butyl-80L 300mL | 300mL | 06132-080100-2300 |
UniHR Butyl-80L 500mL | 500mL | 06132-080100-2500 |
UniHR Butyl-80L 1L | 1L | 06132-080100-1001 |
UniHR Butyl-80L 5L | 5L | 06132-080100-1005 |
UniHR Butyl-80L 10L | 10L | 06132-080100-1010 |
UniHR Butyl-80L 50L | 50L | 06132-080100-1050 |
UniHR Butyl-80L 100L | 100L | 06132-080100-1100 |
UniHR Phenyl-80L 10mL | 10mL | 06131-080100-2010 |
UniHR Phenyl-80L 30mL | 30mL | 06131-080100-2030 |
UniHR Phenyl-80L 50mL | 50mL | 06131-080100-2050 |
UniHR Phenyl-80L 100mL | 100mL | 06131-080100-2100 |
UniHR Phenyl-80L 300mL | 300mL | 06131-080100-2300 |
UniHR Phenyl-80L 500mL | 500mL | 06131-080100-2500 |
UniHR Phenyl-80L 1L | 1L | 06131-080100-1001 |
UniHR Phenyl-80L 5L | 5L | 06131-080100-1005 |
UniHR Phenyl-80L 10L | 10L | 06131-080100-1010 |
UniHR Phenyl-80L 50L | 50L | 06131-080100-1050 |
UniHR Phenyl-80L 100L | 100L | 06131-080100-1100 |
Address：400 W Cummings Park, Suite 5000, Woburn, MA 01801
Phone: +1-(508) 338-3051
Address：1033, Tower A, Ithum Building, plot-A40 Noida Sector 62, Gsutam Buddha Nagar Uttar Pradesh 201301, INDIA
Phone: +91 96548 80558
Address：No. 2, Baichuan street, Suzhou Industrial Park
Phone：400-828-1622
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