• Product description
  • Characteristics
  • Documents
  • Ordering Information

UniSil series HILIC phases have excellent separation effects in multiple modes of hydrophilic interaction chromatography, and allows use of mobile phases containing up to 60% aqueous phase. It is especially suitable for separation of compounds with strong polarity or hydrophilic molecules difficult to retain by conventional reversed phase chromatography. HPLC analysis, preparation and purification of hydrophilic compounds, especially the enhanced retention of strong polarity basic compounds, without use of toxic solvents such as hexane and chloroform. The HILIC chromatography media developed by NanoMicro has high column efficiency, good selectivity, high mechanical strength, high chemical stability, and longer service life. It has strong complementarity to reversed phase chromatography media.


NanoMicro offers a variety of HILIC phases, including non-bonded silica, cyano, amino, diol, amide, diamino and imidazoline bonded phases. In addition, NanoMicro can also customize special HILIC chromatography media for customers to meet special application requirements. Besides silica based HILIC media, NanoMicro also provides polymer-based HILIC chromatography media to meet the requirements of strong acid and base separation conditions.


HILIC-2M has 2-Methyl-Imidazolyl groups. It has good selectivity to polar molecules, strong retention capacity, suitable for the separation of melamine, streptomycin etc.


HILIC-T has Triazolyl groups. It has strong retention for polar compounds, suitable for the separation of oligosaccharides, glycine, etc.


Amide is one of popular HILIC phases. It is more stable bonding phase, suitable for the separation of saccharides, carbohydrates, etc. One of applications is for stevia sugar.

Product Name

UniSil

UniSil

UniSil Diol

UniSil NH2

Matrix

Silica gel

Silica gel

Silica gel

Silica gel

Particle Size (μm)

1.7/2.0/2.7/3/5/5L/8/10/20/30/50

1.7/2.0/2.7/3/5/5L/8/10/20/30/50

3/5/10

3/5/10

Pore Size ( Å)

100

120

120

120

Particle Size CV

5%

5%

5%

5%

Surface Area (m2/g)

~450

~350

~350

~350

Pore Volume (cm3/g)

~0.95

~0.95

~0.95

~0.95

Bulk Density (g/mL)

~0.50

~0.50

~0.50

~0.50

Bonding Phase

None bonded, fully hydrolized

None bonded, fully hydrolized

Diol

Aminopropyl

Carbon Content (%)

~3

~7

~5

Max. Pressure( bar)

600

600

600

600

Working pH

2-8

2-8

2-8

2-8

Operational Temperature ()

10-60

10-60

10-60

10-60

Storage

After use, the media should be cleaned with salt and sealed with high organic phase (100% ethanol or 100% isopropanol) and stored in a cool and dry place. Keep unopened media in a cool and dry place. The recommended storage temperature is 4 ~ 25.


Product Name

UniSil HILIC

UniSil HILIC-2M

UniSil HILIC-T

UniSil Amide

Matrix

Silica gel

Silica gel

Silica gel

Silica gel

Particle Size (μm)

3/5/10

3/5/10

3/5/10

3/5/8/10/20/30/50

Pore Size ( Å)

120

120

120

120

Particle Size CV

5%

5%

5%

5%

Surface Area (m2/g)

~350

~350

~350

~350

Pore Volume (cm3/g)

~0.95

~0.95

~0.95

~0.95

Bulk Density (g/mL)

~0.50

~0.50

~0.50

~0.50

Bonding Phase

Imidazolyl

2-Methylimidazolyl

Triazole

Amide

Carbon Content (%)

~5

~6

~5

~5

Max. Pressure( bar)

600

600

600

600

Working pH

2-8

2-8

2-8

2-8

Operational Temperature ()

10-60

10-60

10-60

10-60

Storage

After use, the media should be cleaned with salt and sealed with high organic phase (100% ethanol or 100% isopropanol) and stored in a cool and dry place. Keep unopened media in a cool and dry place. The recommended storage temperature is 4 ~ 25℃.


We offer products with various particle size and pore size. For detailed ordering information, please contact local sales or leave a message on this website.

Product Name

Cat. No.

Pack Size

UniSil 10-100

19000-100010

100 g, 500g, 1Kg, 5Kg, 10Kg, 20Kg

UniSil 10-120

19000-100012

UniSil 10-120 Diol

19009-100012

UniSil 10-120 NH2

19005-100012

UniSil 10-120 HILIC-T

19012-100012

UniSil 10-120 HILIC-2M

19011-100012

UniSil 10-120 Amide

19017-100012